2015

Pollegioni L, Tonin F, Rosini E. Lignin-degrading enzymes. FEBS J. 2015, 282:1190-1213. doi: 10.1111/febs.13224. Review.

D'Arrigo P, Allegretti C, Fiorati A, Piubelli L, Rosini E, Tessaro D, Valentino M, Pollegioni, L. Immobilization of L-aspartate oxidase from Sulfolobus tokodaii as biocatalyst for resolution of aspartate solutionsCatal Sci Technol. 2015, 5: 1106-1114.  doi: 10.1039/c4cy00968a. 

Conti G, Pollegioni L, Rosini E. One-pot conversion of cephalosporin C by using an optimized two-enzyme process. Catal Sci Technol. 2015, 5:1854-1863. doi: 10.1039/c4cy01522c.

Cappellini F, Recordati C, Maglie M, Pollegioni L, Rossi F, Daturi M, Gornati R, Bernardini G. New synthesis and biodistribution of the D-amino acid oxidase-magnetic nanoparticle system. Future Sci OA. 2015, 1:FSO67. doi: 10.4155/fso.15.67.

Smaldone G, Diana D, Pollegioni L, Di Gaetano S, Fattorusso R, Pedone E. Insight into conformational modification of alpha-synuclein in the presence of neuronal whole cells and of their isolated membranes. FEBS Lett. 2015, 589:798-804. doi: 10.1016/j.febslet.2015.02.012. 

Conti G, Pollegioni L, Rosini E. One-pot conversion of cephalosporin C by using an optimized two-enzyme process. Catal. Sci. Technol. 2015, 5:1854-1863. doi: 10.1039/c4cy01522c.

 

Terry-Lorenzo RT, Masuda K, Sugao K, Fang QK, Orsini MA, Sacchi S, Pollegioni L. High-throughput screening strategy identifies allosteric, covalent human D-amino acid oxidase inhibitor. J Biomol Screen. 2015, 20:1218-1231. doi: 10.1177/1087057115600413. 

Cappelletti P, Piubelli L, Murtas G, Caldinelli L, Valentino M, Molla G, Pollegioni L, Sacchi S. Structure-function relationships in human D-amino acid oxidase variants corresponding to known SNPs. Biochim Biophys Acta. 2015, 1854:1150-1159. doi: 10.1016/j.bbapap.2015.02.005. 

Le Bail M, Martineau M, Sacchi S, Yatsenko N, Radzishevsky I, Conrod S, Ait Ouares K, Wolosker H, Pollegioni L, Billard JM, Mothet JP. Identity of the NMDA receptor coagonist is synapse specific and developmentally regulated in the hippocampus. Proc Natl Acad Sci U S A. 2015, 112: E204-E213. doi: 10.1073/pnas.1416668112. 

Mollica L, Conti G, Pollegioni L, Cavalli A, Rosini E. Unveiling the atomic-level determinants of acylase-ligand complexes: an experimental and computational study. J Chem Inf Model. 2015, 55: 2227-2241. doi: 10.1021/acs.jcim.5b00535. 

Mozzarelli A, Pollegioni L. Special Issue on "Cofactor-dependent proteins: Evolution, chemical diversity and bio-applications". Biochim Biophys Acta. 2015, 1854:1071-1072. doi: 10.1016/j.bbapap.2015.06.001. 

 

2014

Canu N, Ciotti MT, Pollegioni L. Serine racemase: a key player in apoptosis and necrosis. Front Synaptic Neurosci. 2014, 6: 9. doi: 10.3389/fnsyn.2014.00009. Review.

Rosini E, Piubelli L, Molla G, Frattini L, Valentino M, Varriale A, D'Auria S, Pollegioni L. Novel biosensors based on optimized glycine oxidase. FEBS J. 2014, 281: 3460-3472. doi: 10.1111/febs.12873. 

Labarrière M, Thomas F, Dutar P, Pollegioni L, Wolosker H, Billard JM. Circuit-specific changes in D-serine-dependent activation of the N-methyl-D-aspartate receptor in the aging hippocampus. Age (Dordr). 2014, 36:9698. doi: 10.1007/s11357-014-9698-0. 

 

Polcari D, Kwan A, Van Horn MR, Danis L, Pollegioni L, Ruthazer ES, Mauzeroll J. Disk-shaped amperometric enzymatic biosensor for in vivo detection of D-serine. Anal Chem. 2014, 86: 3501-3507. doi: 10.1021/ac404111u. 

Rosini E, Tonin F, Vasylieva N, Marinesco S, Pollegioni L. Evolution of histamine oxidase activity for biotechnological applications. Appl Microbiol Biotechnol. 2014, 98: 739-748. doi: 10.1007/s00253-013-5159-4. 

Conti G, Pollegioni L, Molla G, Rosini E. Strategic manipulation of an industrial biocatalyst--evolution of a cephalosporin C acylase. FEBS J. 2014, 281: 2443-2455. doi: 10.1111/febs.12798. 

Nicolia A, Ferradini N, Molla G, Biagetti E, Pollegioni L, Veronesi F, Rosellini D. Expression of an evolved engineered variant of a bacterial glycine oxidase leads to glyphosate resistance in alfalfa. J Biotechnol. 2014, 184: 201-208. doi: 10.1016/j.jbiotec.2014.05.020. 

 

Terry-Lorenzo RT, Chun LE, Brown SP, Heffernan ML, Fang QK, Orsini MA, Pollegioni L, Hardy LW, Spear KL, Large TH. Novel human D-amino acid oxidase inhibitors stabilize an active-site lid-open conformation. Biosci Rep. 2014, 34:e00133. doi: 10.1042/BSR20140071. 

Molla G, Nardini M, Motta P, D'Arrigo P, Panzeri W, Pollegioni L. Aminoacetone oxidase from Streptococcus oligofermentans belongs to a new three-domain family of bacterial flavoproteins. Biochem J. 2014, 464: 387-399. doi: 10.1042/BJ20140972. 

Lench AM, Massey PV, Pollegioni L, Woodhall GL, Jones RS. Astroglial D-serine is the endogenous co-agonist at the presynaptic NMDA receptor in rat entorhinal cortex. Neuropharmacology. 2014, 83:118-127. doi: 10.1016/j.neuropharm.2014.04.004. 

Temporini C, Bavaro T, Tengattini S, Serra I, Marrubini G, Calleri E, Fasanella F, Piubelli L, Marinelli F, Pollegioni L, Speranza G, Massolini G, Terreni M. Liquid chromatography-mass spectrometry structural characterization of neo glycoproteins aiding the rational design and synthesis of a novel glycovaccine for protection against tuberculosis. J Chromatogr A. 2014,1367: 57-67. doi: 10.1016/j.chroma.2014.09.041. 

Cappelletti P, Campomenosi P, Pollegioni L, Sacchi S. The degradation (by distinct pathways) of human D-amino acid oxidase and its interacting partner pLG72--two key proteins in D-serine catabolism in the brain. FEBS J. 2014, 281: 708-723. doi: 10.1111/febs.12616. 

 

2013

Pollegioni L, Rosini E, Molla G. Cephalosporin C acylase: dream and(/or) reality. Appl Microbiol Biotechnol. 2013, 97:2341-2355. doi: 10.1007/s00253-013-4741-0. Review.

Pollegioni L, Motta P, Molla G. L-amino acid oxidase as biocatalyst: a dream too far? Appl Microbiol Biotechnol. 2013, 97: 9323-9341. doi: 10.1007/s00253-013-5230-1. Review.

Sacchi S, Rosini E, Pollegioni L, Molla G. D-amino acid oxidase inhibitors as a novel class of drugs for schizophrenia therapy. Curr Pharm Des. 2013,19: 2499-2511. doi: 10.2174/1381612811319140002. Review.

Bifulco D, Pollegioni L, Tessaro D, Servi S, Molla G. A thermostable L-aspartate oxidase: a new tool for biotechnological applications. Appl Microbiol Biotechnol. 2013, 97: 7285-7295. doi: 10.1007/s00253-013-4688-1. 

Hopkins SC, Heffernan ML, Saraswat LD, Bowen CA, Melnick L, Hardy LW, Orsini MA, Allen MS, Koch P, Spear KL, Foglesong RJ, Soukri M, Chytil M, Fang QK, Jones SW, Varney MA, Panatier A, Oliet SH, Pollegioni L, Piubelli L, Molla G, Nardini M, Large TH. Structural, kinetic, and pharmacodynamic mechanisms of D-amino acid oxidase inhibition by small molecules. J Med Chem. 2013, 56: 3710-3724. doi: 10.1021/jm4002583. 

Bava A, Gornati R, Cappellini F, Caldinelli L, Pollegioni L, Bernardini G. D-amino acid oxidase-nanoparticle system: a potential novel approach for cancer enzymatic therapy. Nanomedicine (Lond). 2013, 8: 1797-1806. doi: 10.2217/nnm.12.187. 

Maucler C, Pernot P, Vasylieva N, Pollegioni L, Marinesco S. In vivo D-serine hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by microelectrode biosensors. ACS Chem Neurosci. 2013 , 4: 772-781. doi: 10.1021/cn4000549. 

Li Y, Sacchi S, Pollegioni L, Basu AC, Coyle JT, Bolshakov VY. Identity of endogenous NMDAR glycine site agonist in amygdala is determined by synaptic activity level. Nat Commun. 2013, 4: 1760. doi: 10.1038/ncomms2779. 

Piubelli L, Campa M, Temporini C, Binda E, Mangione F, Amicosante M, Terreni M, Marinelli F, Pollegioni L. Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins. Microb Cell Fact. 2013, 12: 115. doi: 10.1186/1475-2859-12-115. 

Caldinelli L, Albani D, Pollegioni L. One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coli. BMC Biotechnol. 2013, 13: 32. doi: 10.1186/1472-6750-13-32. 

Caldinelli L, Sacchi S, Molla G, Nardini M, Pollegioni L. Characterization of human DAAO variants potentially related to an increased risk of schizophrenia. Biochim Biophys Acta. 2013, 1832: 400-410. doi: 10.1016/j.bbadis.2012.11.019.

Binda E, Marcone GL, Berini F, Pollegioni L, Marinelli F. Streptomyces spp. as efficient expression system for a D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance. BMC Biotechnol. 2013, 13: 24. doi: 10.1186/1472-6750-13-24. 

Curcio L, Podda MV, Leone L, Piacentini R, Mastrodonato A, Cappelletti P, Sacchi S, Pollegioni L, Grassi C, D'Ascenzo M. Reduced D-serine levels in the nucleus accumbens of cocaine-treated rats hinder the induction of NMDA receptor-dependent synaptic plasticity. Brain. 2013, 136: 1216-1230. doi: 10.1093/brain/awt036. 

Raimondi I, Ciribilli Y, Monti P, Bisio A, Pollegioni L, Fronza G, Inga A, Campomenosi P. P53 family members modulate the expression of PRODH, but not PRODH2, via intronic p53 response elements. PLoS One. 2013, 8: e69152. doi: 10.1371/journal.pone.0069152. 

Golden E, Paterson R, Tie WJ, Anandan A, Flematti G, Molla G, Rosini E, Pollegioni L, Vrielink A. Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity. Biochem J. 2013, 451: 217-226. doi: 10.1042/BJ20121715. 

Sacchi S. D-Serine metabolism: new insights into the modulation of D-amino acid oxidase activity. Biochem Soc Trans. 2013, 41:1551-1556. doi: 10.1042/BST20130184. Review.

 

2012

Papouin T, Ladépêche L, Ruel J, Sacchi S, Labasque M, Hanini M, Groc L, Pollegioni L, Mothet JP, Oliet SH. Synaptic and extrasynaptic NMDA receptors are gated by different endogenous coagonists. Cell. 2012, 150: 633-646. doi: 10.1016/j.cell.2012.06.029. 

Sacchi S, Caldinelli L, Cappelletti P, Pollegioni L, Molla G. Structure-function relationships in human D-amino acid oxidase. Amino Acids. 2012, 43: 1833-1850. doi: 10.1007/s00726-012-1345-4. Review.

Tedeschi G, Pollegioni L, Negri A. Assays of D-amino acid oxidases. Methods Mol Biol. 2012, 794:381-395. doi: 10.1007/978-1-61779-331-8_26. 

Sacchi S, Rosini E, Caldinelli L, Pollegioni L. Biosensors for D-amino acid detection. Methods Mol Biol. 2012, 794: 313-324. doi: 10.1007/978-1-61779-331-8_21.

Esposito S, Pristerà A, Maresca G, Cavallaro S, Felsani A, Florenzano F, Manni L, Ciotti MT, Pollegioni L, Borsello T, Canu N. Contribution of serine racemase/D-serine pathway to neuronal apoptosis. Aging Cell. 2012, 11: 588-598. doi: 10.1111/j.1474-9726.2012.00822.x. 

Rosini E, Nossa S, Valentino M, D'Arrigo P, Marinesco S, Pollegioni L. Expression of rat diamine oxidase in Escherichia coli J Mol Catal B-Enzym.  2012, 82: 115-120. doi: 10.1016/j.molcatb.2012.06.014.

 

Tallarita E, Pollegioni L, Servi S, Molla G. Expression in Escherichia coli of the catalytic domain of human proline oxidase. Protein Expr Purif. 2012, 82: 345-351. doi: 10.1016/j.pep.2012.01.021. 

Fossat P, Turpin FR, Sacchi S, Dulong J, Shi T, Rivet JM, Sweedler JV, Pollegioni L, Millan MJ, Oliet SH, Mothet JP. Glial D-serine gates NMDA receptors at excitatory synapses in prefrontal cortex. Cereb Cortex. 2012, 22: 595-606. doi: 10.1093/cercor/bhr130. 

Pernot P, Maucler C, Tholance Y, Vasylieva N, Debilly G, Pollegioni L, Cespuglio R, Marinesco S. D-Serine diffusion through the blood-brain barrier: effect on D-serine compartmentalization and storage. Neurochem Int. 2012, 60: 837-845. doi: 10.1016/j.neuint.2012.03.008. 

Binda E, Marcone GL, Pollegioni L, Marinelli F. Characterization of VanYn, a novel D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance in Nonomuraea sp. ATCC 39727. FEBS J. 2012, 279: 3203-3213. doi: 10.1111/j.1742-4658.2012.08706.x. 

Molla G, Piubelli L, Volontè F, Pilone MS. Enzymatic detection of D-amino acids. Methods Mol Biol. 2012, 794: 273-289. doi: 10.1007/978-1-61779-331-8_18. 

 

2011

Pollegioni L, Molla G. New biotech applications from evolved D-amino acid oxidases. Trends Biotechnol. 2011, 29: 276-283. doi: 10.1016/j.tibtech.2011.01.010. Review.

Pollegioni L, Schonbrunn E, Siehl D. Molecular basis of glyphosate resistance-different approaches through protein engineering. FEBS J. 2011, 278: 2753-2766. doi: 10.1111/j.1742-4658.2011.08214.x. Review.

Frattini L, Rosini E, Pollegioni L, Pilone MS. Analyzing the D-amino acid content in biological samples by engineered enzymes. J Chromatogr B Analyt Technol Biomed Life Sci. 2011, 879: 3235-3239. doi: 10.1016/j.jchromb.2011.02.036. 

Volontè F, Pisanelli I, D'Arrigo P, Viani F, Molla G, Servi S, Pollegioni L. Overexpression of a bacterial chymotrypsin: application for L-amino acid ester hydrolysis. Enzyme Microb Technol. 2011, 49: 560-566. doi: 10.1016/j.enzmictec.2011.06.009. 

Volontè F, Piubelli L, Pollegioni L. Optimizing HIV-1 protease production in Escherichia coli as fusion protein. Microb Cell Fact. 2011, 10: 53. doi: 10.1186/1475-2859-10-53. 

Vasylieva N, Barnych B, Meiller A, Maucler C, Pollegioni L, Lin JS, Barbier D, Marinesco S. Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation. Biosens Bioelectron. 2011, 26: 3993-4000. doi: 10.1016/j.bios.2011.03.012. 

Sacchi S, Cappelletti P, Giovannardi S, Pollegioni L. Evidence for the interaction of D-amino acid oxidase with pLG72 in a glial cell line. Mol Cell Neurosci. 2011, 48: 20-28. doi: 10.1016/j.mcn.2011.06.001. 

Rosini E, Molla G, Ghisla S, Pollegioni L. On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity. FEBS J. 2011, 278: 482-492. doi: 10.1111/j.1742-4658.2010.07969.x. 

Ghisla S, Pollegioni L, Molla G. Revisitation of the βCl-elimination reaction of D-amino acid oxidase: new interpretation of the reaction that sparked flavoprotein dehydrogenation mechanisms. J Biol Chem. 2011, 286: 40987-40998. doi: 10.1074/jbc.M111.266536. 

Frattini LF, Piubelli L, Sacchi S, Molla G, Pollegioni L. Is rat an appropriate animal model to study the involvement of D-serine catabolism in schizophrenia? Insights from characterization of D-amino acid oxidase. FEBS J. 2011, 278: 4362-4373. doi: 10.1111/j.1742-4658.2011.08354.x. E

2010

Pollegioni L, Sacchi S. Metabolism of the neuromodulator D-serine. Cell Mol Life Sci. 2010, 67: 2387-2404. doi: 10.1007/s00018-010-0307-9.  Review.

Volontè F, Pollegioni L, Molla G, Frattini L, Marinelli F, Piubelli L. Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli. BMC Biotechnol. 2010, 10: 33. doi: 10.1186/1472-6750-10-33. 

Caldinelli L, Molla G, Bracci L, Lelli B, Pileri S, Cappelletti P, Sacchi S, Pollegioni L. Effect of ligand binding on human D-amino acid oxidase: implications for the development of new drugs for schizophrenia treatment. Protein Sci. 2010, 19: 1500-1512. doi: 10.1002/pro.429. 

Saam J, Rosini E, Molla G, Schulten K, Pollegioni L, Ghisla S. O2 reactivity of flavoproteins: dynamic access of dioxygen to the active site and role of a H+ relay system in D-amino acid oxidase. J Biol Chem. 2010, 285:  24439-24446. doi: 10.1074/jbc.M110.131193. 

2009

Pollegioni L, Piubelli L, Molla G. Cholesterol oxidase: biotechnological applications. FEBS J. 2009, 276: 6857-6870. doi: 10.1111/j.1742-4658.2009.07379.x.  Review.

Pedotti M, Rosini E, Molla G, Moschetti T, Savino C, Vallone B, Pollegioni L. Glyphosate resistance by engineering the flavoenzyme glycine oxidase. J Biol Chem. 2009, 284: 36415-36423. doi: 10.1074/jbc.M109.051631. 

Caldinelli L, Pedotti M, Motteran L, Molla G, Pollegioni L. FAD binding in glycine oxidase from Bacillus subtilis. Biochimie. 2009, 91: 1499-1508. doi: 10.1016/j.biochi.2009.09.003. 

Pollegioni L. Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool. FEBS J. 2009, 276: 6825. doi: 10.1111/j.1742-4658.2009.07376.x. 

Romano D, Molla G, Pollegioni L, Marinelli F. Optimization of human D-amino acid oxidase expression in Escherichia coli. Protein Expr Purif. 2009, 68: 72-78. doi: 10.1016/j.pep.2009.05.013.

Caldinelli L, Molla G, Sacchi S, Pilone MS, Pollegioni L. Relevance of weak flavin binding in human D-amino acid oxidase. Protein Sci. 2009, 18: 801-810. doi: 10.1002/pro.86. 

Pedotti M, Ghisla S, Motteran L, Molla G, Pollegioni L. Catalytic and redox properties of glycine oxidase from Bacillus subtilis. Biochimie. 2009, 91: 604-612. doi: 10.1016/j.biochi.2009.02.007. 

Rosini E, Pollegioni L, Ghisla S, Orru R, Molla G. Optimization of D-amino acid oxidase for low substrate concentrations--towards a cancer enzyme therapy. FEBS J. 2009, 276: 4921-4932. doi: 10.1111/j.1742-4658.2009.07191.x. 

Donini S, Ferrari M, Fedeli C, Faini M, Lamberto I, Marletta AS, Mellini L, Panini M, Percudani R, Pollegioni L, Caldinelli L, Petrucco S, Peracchi A. Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism. Biochem J. 2009, 422: 265-272. doi: 10.1042/BJ20090748. 

2008

Pollegioni L, Molla G, Sacchi S, Rosini E, Verga R, Pilone MS. Properties and applications of microbial D-amino acid oxidases: current state and perspectives. Appl Microbiol Biotechnol. 2008, 78: 1-16.  doi: 10.1007/s00253-007-1282-4.  Review.

Volontè F, Marinelli F, Gastaldo L, Sacchi S, Pilone MS, Pollegioni L, Molla G. Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli. Protein Expr Purif. 2008 , 61: 131-137. doi: 10.1016/j.pep.2008.05.010. 

Rosini E, Molla G, Rossetti C, Pilone MS, Pollegioni L, Sacchi S. A biosensor for all D-amino acids using evolved D-amino acid oxidase. J Biotechnol. 2008, 135: 377-384. doi: 10.1016/j.jbiotec.2008.06.001. 

Sacchi S, Bernasconi M, Martineau M, Mothet JP, Ruzzene M, Pilone MS, Pollegioni L, Molla G. pLG72 modulates intracellular D-serine levels through its interaction with D-amino acid oxidase: effect on schizophrenia susceptibility. J Biol Chem. 2008, 283: 22244-22256. doi: 10.1074/jbc.M709153200. 

Pernot P, Mothet JP, Schuvailo O, Soldatkin A, Pollegioni L, Pilone M, Adeline MT, Cespuglio R, Marinesco S. Characterization of a yeast D-amino acid oxidase microbiosensor for D-serine detection in the central nervous system. Anal Chem. 2008, 80: 1589-1597. doi: 10.1021/ac702230w. 

Caldinelli L, Iametti S, Barbiroli A, Fessas D, Bonomi F, Piubelli L, Molla G, Pollegioni L. Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD. Protein Sci. 2008, 17: 409-419. doi: 10.1110/ps.073137708. 

Piubelli L, Pedotti M, Molla G, Feindler-Boeckh S, Ghisla S, Pilone MS, Pollegioni L. On the oxygen reactivity of flavoprotein oxidases: an oxygen access tunnel and gate in Brevibacterium sterolicum cholesterol oxidase. J Biol Chem. 2008, 283: 24738-24747. doi: 10.1074/jbc.M802321200. 

2007

Pollegioni L, Sacchi S, Caldinelli L, Boselli A, Pilone MS, Piubelli L, Molla G. Engineering the properties of D-amino acid oxidases by a rational and a directed evolution approach. Curr Protein Pept Sci. 2007, 8: 600-618. doi: 10.2174/138920307783018677. Review.

Boselli A, Rosini E, Marcone GL, Sacchi S, Motteran L, Pilone MS, Pollegioni L, Molla G. Glycine oxidase from Bacillus subtilis: role of histidine 244 and methionine 261. Biochimie. 2007, 89:1372-80. doi: 10.1016/j.biochi.2007.04.019. 

Boselli A, Piubelli L, Molla G, Pilone MS, Pollegioni L, Sacchi S. Investigating the role of active site residues of Rhodotorula gracilis D-amino acid oxidase on its substrate specificity. Biochimie. 2007, 89: 360-368. doi: 10.1016/j.biochi.2006.10.017. 

Kay CW, El Mkami H, Molla G, Pollegioni L, Ramsay RR. Characterization of the covalently bound anionic flavin radical in monoamine oxidase a by electron paramagnetic resonance. J Am Chem Soc. 2007, 129: 16091-16097. doi: 10.1021/ja076090q.

Pollegioni L, Piubelli L, Sacchi S, Pilone MS, Molla G. Physiological functions of D-amino acid oxidases: from yeast to humans. Cell Mol Life Sci. 2007, 64: 1373-1394. doi: 10.1007/s00018-007-6558-4. Review.

2006

Lim L, Molla G, Guinn N, Ghisla S, Pollegioni L, Vrielink A. Structural and kinetic analyses of the H121A mutant of cholesterol oxidase. Biochem J. 2006, 400: 13-22. doi: 10.1042/BJ20060664. 

Panatier A, Theodosis DT, Mothet JP, Touquet B, Pollegioni L, Poulain DA, Oliet SH. Glia-derived D-serine controls NMDA receptor activity and synaptic memory. Cell. 2006, 125: 775-784. doi: 10.1016/j.cell.2006.02.051. 

Molla G, Bernasconi M, Sacchi S, Pilone MS, Pollegioni L. Expression in Escherichia coli and in vitro refolding of the human protein pLG72. Protein Expr Purif. 2006, 46: 150-155. doi: 10.1016/j.pep.2005.08.003. 

Caldinelli L, Molla G, Pilone MS, Pollegioni L. Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis. FEBS J. 2006, 273: 504-512. doi: 10.1111/j.1742-4658.2005.05083.x. 

Molla G, Sacchi S, Bernasconi M, Pilone MS, Fukui K, Pollegioni L. Characterization of human D-amino acid oxidase. FEBS Lett. 2006, 580: 2358-2364. doi: 10.1016/j.febslet.2006.03.045. 

2005

Pollegioni L, Lorenzi S, Rosini E, Marcone GL, Molla G, Verga R, Cabri W, Pilone MS. Evolution of an acylase active on cephalosporin C. Protein Sci. 2005, 14: 3064-3076. doi: 10.1110/ps.051671705. 

Mothet JP, Pollegioni L, Ouanounou G, Martineau M, Fossier P, Baux G. Glutamate receptor activation triggers a calcium-dependent and SNARE protein-dependent release of the gliotransmitter D-serine. Proc Natl Acad Sci U S A. 2005, 102: 5606-5611. doi: 10.1073/pnas.0408483102. 

Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Fessas D, Molla G, Pilone MS, Pollegioni L. Dissecting the structural determinants of the stability of cholesterol oxidase containing covalently bound flavin. J Biol Chem. 2005, 280: 22572-22581. doi: 10.1074/jbc.M500549200. 

2004

Mörtl M, Diederichs K, Welte W, Molla G, Motteran L, Andriolo G, Pilone MS, Pollegioni L. Structure-function correlation in glycine oxidase from Bacillus subtilis. J Biol Chem. 2004, 279: 29718-29727. doi: 10.1074/jbc.M401224200. 

Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Piubelli L, Ferranti P, Picariello G, Pilone MS, Pollegioni L. Unfolding intermediate in the peroxisomal flavoprotein D-amino acid oxidase. J Biol Chem. 2004, 279: 28426-28434. doi: 10.1074/jbc.M403489200. 

Pollegioni L, Caldinelli L, Molla G, Sacchi S, Pilone MS. Catalytic properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison between proteins from different sources. Biotechnol Prog. 2004, 20: 467-473. doi: 10.1021/bp034206q. 

Boselli A, Piubelli L, Molla G, Sacchi S, Pilone MS, Ghisla S, Pollegioni L. On the mechanism of Rhodotorula gracilis D-amino acid oxidase: role of the active site serine 335. Biochim Biophys Acta. 2004, 1702: 19-32. doi: 10.1016/j.bbapap.2004.07.005. 

Sacchi S, Rosini E, Molla G, Pilone MS, Pollegioni L. Modulating D-amino acid oxidase substrate specificity: production of an enzyme for analytical determination of all D-amino acids by directed evolution. Protein Eng Des Sel. 2004, 17: 517-525. doi: 10.1093/protein/gzh064. 

2003

Molla G, Motteran L, Job V, Pilone MS, Pollegioni L. Kinetic mechanisms of glycine oxidase from Bacillus subtilis. Eur J Biochem. 2003, 270: 1474-1482. doi: 10.1046/j.1432-1033.2003.03513.x. 

Molla G, Motteran L, Piubelli L, Pilone MS, Pollegioni L. Regulation of D-amino acid oxidase expression in the yeast Rhodotorula gracilis. Yeast. 2003, 20: 1061-1069. doi: 10.1002/yea.1023. 

Pollegioni L, Iametti S, Fessas D, Caldinelli L, Piubelli L, Barbiroli A, Pilone MS, Bonomi F. Contribution of the dimeric state to the thermal stability of the flavoprotein D-amino acid oxidase. Protein Sci. 2003, 12: 1018-1029. doi: 10.1110/ps.0234603. 

Piubelli L, Molla G, Caldinelli L, Pilone MS, Pollegioni L. Dissection of the structural determinants involved in formation of the dimeric form of D-amino acid oxidase from Rhodotorula gracilis: role of the size of the betaF5-betaF6 loop. Protein Eng. 2003, 16: 1063-1069. doi: 10.1093/protein/gzg125. 

2002

Sacchi S, Lorenzi S, Molla G, Pilone MS, Rossetti C, Pollegioni L. Engineering the substrate specificity of D-amino-acid oxidase. J Biol Chem. 2002, 277: 27510-27516. doi: 10.1074/jbc.M203946200. 

Job V, Marcone GL, Pilone MS, Pollegioni L. Glycine oxidase from Bacillus subtilis. Characterization of a new flavoprotein. J Biol Chem. 2002, 277: 6985-6993. doi: 10.1074/jbc.M111095200. 

Job V, Molla G, Pilone MS, Pollegioni L. Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli. Eur J Biochem. 2002, 269: 1456-1463. doi: 10.1046/j.1432-1033.2002.02790.x. 

Piubelli L, Caldinelli L, Molla G, Pilone MS, Pollegioni L. Conversion of the dimeric D-amino acid oxidase from Rhodotorula gracilis to a monomeric form. A rational mutagenesis approach. FEBS Lett. 2002, 526: 43-48. doi: 10.1016/s0014-5793(02)03111-3. 

Ardini E, Sporchia B, Pollegioni L, Modugno M, Ghirelli C, Castiglioni F, Tagliabue E, Ménard S. Identification of a novel function for 67-kDa laminin receptor: increase in laminin degradation rate and release of motility fragments. Cancer Res. 2002, 62: 1321-1325. 

Vigetti D, Pollegioni L, Monetti C, Prati M, Bernardini G, Gornati R. Property comparison of recombinant amphibian and mammalian allantoicases. FEBS Lett. 2002, 512: 323-328. doi: 10.1016/s0014-5793(02)02264-0. 

Boselli A, Sacchi S, Job V, Pilone MS, Pollegioni L. Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study. Eur J Biochem. 2002, 269: 4762-4771. doi: 10.1046/j.1432-1033.2002.t01-1-03173.x. 

Pollegioni L, Diederichs K, Molla G, Umhau S, Welte W, Ghisla S, Pilone MS. Yeast D-amino acid oxidase: structural basis of its catalytic properties. J Mol Biol. 2002, 324: 535-546. doi: 10.1016/s0022-2836(02)01062-8. 

2001

Harris CM, Pollegioni L, Ghisla S. pH and kinetic isotope effects in D-amino acid oxidase catalysis. Eur J Biochem. 2001, 268: 5504-5520. doi: 10.1046/j.1432-1033.2001.02462.x. 

Motteran L, Pilone MS, Molla G, Ghisla S, Pollegioni L. Cholesterol oxidase from Brevibacterium sterolicum. The relationship between covalent flavinylation and redox properties. J Biol Chem. 2001, 276: 18024-18030. doi: 10.1074/jbc.M010953200. 

Pollegioni L, Harris CM, Molla G, Pilone MS, Ghisla S. Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase. FEBS Lett. 2001, 507: 323-326. doi: 10.1016/s0014-5793(01)02983-0. 

 

 

2000

Molla G, Porrini D, Job V, Motteran L, Vegezzi C, Campaner S, Pilone MS, Pollegioni L. Role of arginine 285 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study. J Biol Chem. 2000, 275: 24715-24721. doi: 10.1074/jbc.M908193199. 

Vigetti D, Monetti C, Pollegioni L, Taramelli R, Bernardini G. Xenopus allantoicase: molecular cloning, enzymatic activity and developmental expression. Arch Biochem Biophys. 2000, 379: 90-96. doi: 10.1006/abbi.2000.1863. 

Umhau S, Pollegioni L, Molla G, Diederichs K, Welte W, Pilone MS, Ghisla S. The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation. Proc Natl Acad Sci U S A. 2000, 97: 12463-12468. doi: 10.1073/pnas.97.23.12463. 

Pollegioni L, Porrini D, Molla G, Pilone MS. Redox potentials and their pH dependence of D-amino-acid oxidase of Rhodotorula gracilis and Trigonopsis variabilis. Eur J Biochem. 2000, 267: 6624-6632. doi: 10.1046/j.1432-1327.2000.01757.x. 

Pilone MS. D-Amino acid oxidase: new findings. Cell Mol Life Sci. 2000, 57: 1732-1747. doi: 10.1007/PL00000655. Review.

1999

Harris CM, Molla G, Pilone MS, Pollegioni L. Studies on the reaction mechanism of Rhodotorula gracilis D-amino-acid oxidase. Role of the highly conserved Tyr-223 on substrate binding and catalysis. J Biol Chem. 1999, 274: 36233-36240. doi: 10.1074/jbc.274.51.36233. 

Pollegioni L, Gadda G, Ambrosius D, Ghisla S, Pilone MS. Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum: effect of surfactants and organic solvents on activity. Biotechnol Appl Biochem. 1999, 30: 27-33. 

Pollegioni L, Wels G, Pilone MS, Ghisla S. Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum. Eur J Biochem. 1999, 264: 140-151. doi: 10.1046/j.1432-1327.1999.00586.x.