OCTOBER 2021: 

new review available on anticancer therapy based on oxidases that produce reactive oxygen species.

Rosini and Pollegioni, 2021. Biofactors. doi: 10.1002/biof.1789.

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SEPTEMBER 2021:

New publication:

 

Vignali et al., 2021. Advanced Synthesis & Catalysis. doi.org/10.1002/adsc.202100849.                                                                                                           

in this work, we reported the first multi-enzymatic one-pot bioconversion of vanillin into cis,cis-muconic acid (ccMA), which is widely used for the production of biodegradable plastic materials. Our proposed biocatalytic system represents a sustainable alternative for the eco-friendly production of a high value-added compound from underutilized lignin, a primary target of the bioeconomy.                                                                                          

JULY 2021: 

Professor Loredano Pollegioni has been elected Treasurer of the International Union of Biochemistry and Molecular Biology.

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Antibody-drug conjugates, also named “armed antibodies”, can direct drugs to specific tissues. Here an evolved version of the ROS-generating enzyme D-amino acid oxidase was delivered to tumors by generating a chimeric protein with the F8 antibody against EDA of fibronectin, a protein abundantly expressed in the subendothelial extracellular matrix of tumor vessels. The recombinant chimeric protein produced in E. coli cells generates a strong cytotoxicity to tumor cells following the supplementation of the pro-drug D-alanine, especially when an inhibitor of the antioxidant systems is used.

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APRIL 2021:

New publication:

the human enzyme D-3-phosphoglycerate dehydrogenase catalyzes the first step of the L-serine biosynthesis, which plays an important role in numerous physiological functions. This enzyme is involved in cancer progression and several neurological disorders. Here is our new publication on PHGDH biochemical properties, part of the PRIN project “Dissecting serine metabolism in the brain”. https://www.mdpi.com/1422-0067/22/8/4231/htm

The cover art by Valentina Pirillo has been selected in The FEBS Journal Cover Competition 2021:   

this image is an artist representation of a plastic-degrading enzyme (PET hydrolase) that is correlated with the recent Emerging Methods and Technologies article published in The FEBS Journal: ‘Analytical methods for the investigation of enzyme-catalyzed degradation of polyethylene terephthalate’ by Valentina Pirillo, Loredano Pollegioni and Gianluca Molla.       

                                                                                                                                 

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JANUARY 2021:

NOVEMBER 2020:

Elena Rosini produced the recombinant SARS-CoV-2 Spike (S) protein. The coronavirus spike protein plays a key role in the early steps of COVID-19 viral infection, mainly through the interaction with the human angiotensin-converting enzyme 2 (ACE2) present on the surface of alveolar epithelial cells. Both the full-length and a truncated form (containing only the receptor binding domain) of S-protein are available at "The Protein Factory 2.0" lab and can be used to develop new diagnostic tools.

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