Rosini E, Pollegioni L. Optimized rapid production of recombinant secreted proteins in CHO cells grown in suspension: The case of RBD. Biotechnol. Appl. Biochem. 2022 Oct 3. doi: 10.1002/bab.2409. Epub ahead of print. 


Maffioli E, Murtas G, Rabattoni V, Badone B, Tripodi F, Iannuzzi F, Licastro D, Nonnis S, Rinaldi AM, Motta Z, Sacchi S, Canu N, Tedeschi G, Coccetti P, Pollegioni L. Insulin and serine metabolism as sex-specific hallmarks of Alzheimer’s disease in the human hippocampus. Cell Reports 2022, Volume 40, Issue 10, 111271, https://doi.org/10.1016/j.celrep.2022.111271.

Vignali E, Gigli M, Cailotto S, Pollegioni L, Rosini E, Crestini C. The laccase-Lig multienzymatic multistep system in lignin valorization. Chem. Sus. Chem. 2022 Aug 2:e202201147. doi: 10.1002/cssc.202201147. Epub ahead of print. 


Giangeri G, Morlino MS, De Bernardini N, Ji M, Bosaro M, Pirillo V, Antoniali P, Molla G, Raga R, Treu L, Campanaro S. Preliminary investigation of microorganisms potentially involved in microplastics degradation using an integrated metagenomic and biochemical approach. Sci. Total Environ. 2022 Jun 28;843:157017. doi: 10.1016/j.scitotenv.2022.157017. Epub ahead of print. 


Murtas G, Pollegioni L, Molla G, Sacchi S. Biochemical properties and physiological functions of pLG72: Twenty years of investigations. Biomolecules 2022 Jun 20;12(6):858. doi: 10.3390/biom12060858. Review 


Pollegioni L, Molla G. The conundrum in enzymatic reactions related to biosynthesis of D-amino acids in bacteria. FEBS J. 2022 May 19. doi: 10.1111/febs.16475. Epub ahead of print. 


Boreggio M, Rosini E, Gambarotti C, Pollegioni L, Fasoli E. Unveiling the bio-corona fingerprinting of potential anticancer carbon nanotubes coupled with D-amino acid oxidase. Mol. Biotechnol. 2022, Oct;64(10):1164-1176. doi: 10.1007/s12033-022-00488-y. Epub 2022 Apr 25. 


Pirillo V, Orlando M, Tessaro D, Pollegioni L, Molla G. An efficient protein evolution workflow for the improvement of bacterial PET hydrolyzing enzymes. Int. J. Mol. Sci. 2022, 23(1), 264. doi: 10.3390/ijms23010264.




Moussa S, Rosini E, Chitsaz D, Pollegioni L, Kennedy TE, Mauzeroll J. High-throughput strategy for glycine oxidase biosensor development reveals glycine release from cultured cells. Anal Chem. 2021. Dec 14;93(49):16504-16511. doi: 10.1021/acs.analchem.1c03620. Epub 2021 Nov 29.


Murtas G, Pollegioni L. D-amino acids as novel blood-based biomarkers. Curr Med Chem. 2021. 29(24):4202-4215. doi: 10.2174/0929867328666211125092438.  Review 


Rosini E, Pollegioni L. Reactive oxygen species as a double-edged sword: the role of oxidative enzymes in antitumor therapy. Biofactors. 2022. Mar;48(2):384-399. doi: 10.1002/biof.1789. Epub 2021 Oct 4. Review 


Vignali E, Pollegioni L, Di Nardo G, Valetti F, Gazzola S, Gilardi G, Rosini E. Multi-enzymatic cascade reactions for the synthesis of cis,cis-muconic acid. Adv. Synth.Catal. 2021. doi: 10.1002/adsc.202100849.


Moussa S, Murtas G, Pollegioni L, Mauzeroll J. Enhancing electrochemical biosensor selectivity with engineered D-amino acid oxidase enzymes for D-serine and D-alanine quantificationACS Appl. Bio Mater. 2021.  doi: 10.1021/acsabm.1c00409.


Pollegioni L, Molla G, Sacchi S, Murtas G. Human D-aspartate oxidase: a key player in D-aspartate metabolism. Front Mol Biosci. 2021, 8:689719. doi: 10.3389/fmolb.2021.689719. Review 


Rosini E, Volpi NA, Ziffels B, Grimaldi A, Sacchi S, Neri D, Pollegioni L. An antibody-based enzymatic therapy for cancer treatment: The selective localization of D-amino acid oxidase to EDA fibronectin. Nanomedicine. 2021 Aug;36:102424. doi: 10.1016/j.nano.2021.102424. Epub 2021 Jun 24. 


Dallérac G, Li X, Lecouflet P, Morisot N, Sacchi S, Asselot R, Pham TH, Potier B, Watson DJG, Schmidt S, Levasseur G, Fossat P, Besedin A, Rivet JM, Coyle JT, Collo G, Pollegioni L, Kehr J, Galante M, Fone KC, Gardier AM, Freret T, Contarino A, Millan MJ, Mothet JP. Dopaminergic neuromodulation of prefrontal cortex activity requires the NMDA receptor coagonist D-serine. Proc Natl Acad Sci U S A. 2021, 118(23):e2023750118. doi: 10.1073/pnas.2023750118. 


Sacchi S, Rabattoni V, Miceli M, Pollegioni L. Yin and Yang in post-translational modifications of human D-amino acid oxidase. Front Mol Biosci. 2021 May 10;8:684934. doi: 10.3389/fmolb.2021.684934. 

Murtas G, Marcone GL, Peracchi A, Zangelmi E, Pollegioni, L. Biochemical and biophysical characterization of recombinant human 3-phosphoglycerate dehydrogenase. Int. J. Mol. Sci. 2021, 22, 4231. https://doi.org/10.3390/ijms22084231.


Pirillo V, Pollegioni L, Molla G. Analytical methods for the investigation of enzyme-catalyzed degradation of polyethylene terephthalate. FEBS J. 2021 Aug;288(16):4730-4745. doi: 10.1111/febs.15850. Epub 2021 May 14. 


Moussa S, Chhin D, Pollegioni L, Mauzeroll J. Quantitative measurements of free and immobilized RgDAAO Michaelis-Menten constant using an electrochemical assay reveal the impact of covalent cross-linking on substrate specificity. Anal Bioanal Chem. 2021 Nov;413(27):6793-6802. doi: 10.1007/s00216-021-03273-z. Epub 2021 Apr 1.


Rabattoni V, Pollegioni L, Tedeschi G, Maffioli E, Sacchi S. Cellular studies of the two main isoforms of human D-aspartate oxidase. FEBS J. 2021 Aug;288(16):4939-4954. doi: 10.1111/febs.15797. Epub 2021 Mar 30. 


Moussa S, Van Horn M R, Shah A, Pollegioni L, Thibodeaux C J, Ruthazer E S, Mauzeroll J. Editors' Choice—A Miniaturized Enzymatic Biosensor for Detection of Sensory-Evoked D-serine Release in the BrainJ. Electrochem. Soc. 2021,168, 025502.


Piubelli L, Murtas G, Rabattoni V, Pollegioni L. The role of D-amino acids in Alzheimer’s disease. J Alzheimers Dis. 2021, 80:475-492. doi: 10.3233/JAD-201217. Review 


Piubelli L, Pollegioni L, Rabattoni V, Mauri M, Princiotta-Cariddi L, Versino M, Sacchi S. Serum D-serine levels are altered in early phases of Alzheimer's desease: towards a precocious biomarkerTransl Psych. 2021, 11:77. doi: org/10.1038/s41398-021-01202-3.


Murtas G, Sacchi S, Tedeschi G, Maffioli E, Notomista E, Cafaro V, Abbondi M, Mothet JP, Pollegioni L. Antimicrobial D-amino acid oxidase-derived peptides specify gut microbiota. Cell Mol Life Sci. 2021, 78:3607-3620. doi: 10.1007/s00018-020-03755-w.


Brennenstuhl H, Didiasova M, Assmann B, Bertoldi M, Molla G, Jung-Klawitter S, Kuseyri Hübschmann O, Schröter J, Opladen T, Tikkanen R. Succinic semialdehyde dehydrogenase deficiency: in vitro and in silico characterization of a novel pathogenic missense variant and analysis of the mutational spectrum of ALDH5A1. Int J Mol Sci. 2020, 21: 8578. doi: 10.3390/ijms21228578. 


Marcone GL, Rosini E, Crespi E, Pollegioni L. D-amino acids in foods. Appl Microbiol Biotechnol. 2020, 104:555-574. doi: 10.1007/s00253-019-10264-9.Review 


Pollegioni L, Rosini E, Molla G. Advances in enzymatic synthesis of D-amino acids. Int J Mol Sci. 2020, 21: 3206. doi: 10.3390/ijms21093206. Review 


Rosini E, Pollegioni L. PEG-DAAO conjugate: a promising tool for cancer therapy optimized by protein engineering. Nanomedicine. 2020, 24:102122. doi: 10.1016/j.nano.2019.102122. 

Molla G, Chaves-Sanjuan A, Savinelli A, Nardini M, Pollegioni L. Structure and kinetic properties of human D-aspartate oxidase, the enzyme-controlling D-aspartate levels in brain. FASEB J. 2020, 34:1182-1197. doi: 10.1096/fj.201901703R. 


Rosini E, D'Antona P, Pollegioni L. Biosensors for D-amino acids: detection methods and applications. Int J Mol Sci. 2020, 21: 4574. doi: 10.3390/ijms21134574. Review 

Carenzi G, Sacchi S, Abbondi M, Pollegioni L. Direct chromatographic methods for enantioresolution of amino acids: recent developments. Amino Acids. 2020, 52: 849-862. doi: 10.1007/s00726-020-02873-w. Review 

Murtas G, Marcone GL, Sacchi S, Pollegioni L. L-serine synthesis via the phosphorylated pathway in humans. Cell Mol Life Sci. 2020. Dec;77(24):5131-5148. doi: 10.1007/s00018-020-03574-z. Epub 2020 Jun 27. Review 


Puggioni V, Savinelli A, Miceli M, Molla G, Pollegioni L, Sacchi S. Biochemical characterization of mouse D-aspartate oxidase. Biochim Biophys Acta Proteins Proteom. 2020, 1868:140472. doi: 10.1016/j.bbapap.2020.140472. 

Tomasello G, Armenia I, Molla G. The Protein Imager: a full-featured online molecular viewer interface with server-side HQ-rendering capabilities. Bioinformatics. 2020, 36:2909-2911. doi: 10.1093/bioinformatics/btaa009. 


Rosini E, De Vito A, Ponti F, Scaldaferri D, Pollegioni L, Acquati F. Expression and purification of the human tumor suppressor protein RNASET2 in CHO-S cells. Protein Expr Purif. 2020, 174: 105675. doi: 10.1016/j.pep.2020.105675. 

La Vitola P, Balducci C, Baroni M, Artioli L, Santamaria G, Castiglioni M, Cerovic M, Colombo L, Caldinelli L, Pollegioni L, Forloni G. Peripheral inflammation exacerbates α-synuclein toxicity and neuropathology in Parkinson's models. Neuropathol Appl Neurobiol. 2020. Feb;47(1):43-60. doi: 10.1111/nan.12644. Epub 2020 Aug 6. 

Murtas G, Sacchi S, Kumar MS, Bosco DA, Pollegioni L. Is the primate-specific protein pLG72 affecting SOD1 functionality and superoxide formation? Free Radic Res. 2020, 54: 419-430. doi: 10.1080/10715762.2020.1791335. 

Loi M, Renaud JB, Rosini E, Pollegioni L, Vignali E, Haidukowski M, Sumarah MW, Logrieco AF, Mulè G. Enzymatic transformation of aflatoxin B1 by Rh_DypB peroxidase and characterization of the reaction products. Chemosphere. 2020, 250: 126296. doi: 10.1016/j.chemosphere.2020.126296. 

Deng X, Zhang Y, Chen Z, Kumata K, Van R, Rong J, Shao T, Hatori A, Mori W, Yu Q, Hu K, Fujinaga M, Wey HY, Shao Y, Josephson L, Murtas G, Pollegioni L, Zhang MR, Liang S. Synthesis and preliminary evaluation of 4-hydroxy-6-(3-[11C]methoxyphenethyl)pyridazin-3(2H)-one, a 11C-labeled d-amino acid oxidase (DAAO) inhibitor for PET imaging. Bioorg Med Chem Lett. 2020, 30: 127326. doi: 10.1016/j.bmcl.2020.127326. 

Baranzini N, De Vito A, Orlandi VT, Reguzzoni M, Monti L, de Eguileor M, Rosini E, Pollegioni L, Tettamanti G, Acquati F, Grimaldi A. Antimicrobial role of RNASET2 protein during innate immune response in the medicinal leech Hirudo verbana. Front Immunol. 2020, 11: 370. doi: 10.3389/fimmu.2020.00370. 


Mothet JP, Billard JM, Pollegioni L, Coyle JT, Sweedler JV. Investigating brain D-serine: advocacy for good practices. Acta Physiol (Oxf). 2019, 226: e13257. doi: 10.1111/apha.13257. Review.

Nuzzo T, Punzo D, Devoto P, Rosini E, Paciotti S, Sacchi S, Li Q, Thiolat ML, Véga C, Carella M, Carta M, Gardoni F, Calabresi P, Pollegioni L, Bezard E, Parnetti L, Errico F, Usiello A. The levels of the NMDA receptor co-agonist D-serine are reduced in the substantia nigra of MPTP-lesioned macaques and in the cerebrospinal fluid of Parkinson's disease patients. Sci Rep. 2019, 9: 8898. doi: 10.1038/s41598-019-45419-1. 

Motta P, Pollegioni L, Molla G. Properties of L-amino acid deaminase: en route to optimize bioconversion reactions. Biochimie. 2019, 158:199-207. doi: 10.1016/j.biochi.2019.01.010. 

Nuzzo T, Feligioni M, Cristino L, Pagano I, Marcelli S, Iannuzzi F, Imperatore R, D'Angelo L, Petrella C, Carella M, Pollegioni L, Sacchi S, Punzo D, De Girolamo P, Errico F, Canu N, Usiello A. Free D-aspartate triggers NMDA receptor-dependent cell death in primary cortical neurons and perturbs JNK activation, Tau phosphorylation, and protein SUMOylation in the cerebral cortex of mice lacking D-aspartate oxidase activity. Exp Neurol. 2019, 317: 51-65. doi: 10.1016/j.expneurol.2019.02.014. 

Marcone GL, Binda E, Rosini E, Abbondi M, Pollegioni L. Antibacterial properties of D-amino acid oxidase: impact on the food industry. Front Microbiol. 2019, 10: 2786. doi: 10.3389/fmicb.2019.02786. 

Rinaldi F, Lupu L, Rusche H, Kukačka Z, Tengattini S, Bernardini R, Piubelli L, Bavaro T, Maeser S, Pollegioni L, Calleri E, Przybylski M, Temporini C. Epitope and affinity determination of recombinant Mycobacterium tuberculosis Ag85B antigen towards anti-Ag85 antibodies using proteolytic affinity-mass spectrometry and biosensor analysis. Anal Bioanal Chem. 2019, 411: 439-448. doi: 10.1007/s00216-018-1466-z. 

Elfgen A, Hupert M, Bochinsky K, Tusche M, González de San Román Martin E, Gering I, Sacchi S, Pollegioni L, Huesgen PF, Hartmann R, Santiago-Schübel B, Kutzsche J, Willbold D. Metabolic resistance of the D-peptide RD2 developed for direct elimination of amyloid-β oligomers. Sci Rep. 2019, 9: 5715. doi: 10.1038/s41598-019-41993-6. 

Pirone L, Caldinelli L, Di Lascio S, Di Girolamo R, Di Gaetano S, Fornasari D, Pollegioni L, Benfante R, Pedone E. Molecular insights into the role of the polyalanine region in mediating PHOX2B aggregation. FEBS J. 2019, 286: 2505-2521. doi: 10.1111/febs.14841. 

La Vitola P, Beeg M, Balducci C, Santamaria G, Restelli E, Colombo L, Caldinelli L, Pollegioni L, Gobbi M, Chiesa R, Forloni G. Cellular prion protein neither binds to alpha-synuclein oligomers nor mediates their detrimental effects. Brain. 2019, 142: 249-254. doi: 10.1093/brain/awy318. 

Murtas G, Sacchi S, Pollegioni L. Substitution of arginine 120 in human D-amino acid oxidase favors FAD-binding and nuclear mistargeting. Front Mol Biosci. 2019, 6: 125. doi: 10.3389/fmolb.2019.00125. 

Vismara E, Bernardi A, Bongio C, Farè S, Pappalardo S, Serafini A, Pollegioni L, Rosini E, Torri G. Bacterial nanocellulose and its surface modification by glycidyl methacrylate and ethylene glycol dimethacrylate. Incorporation of vancomycin and ciprofloxacin. Nanomaterials (Basel). 2019, 9: 1668. doi: 10.3390/nano9121668. 

Rosini E, Pollegioni L. A comprehensive practical laboratory course on protein engineering: evolution of a glycine oxidase variant active on the herbicide glyphosate. Biochem Mol Biol Educ. 2019, 47: 689-699. doi: 10.1002/bmb.21291. 



Pollegioni L, Sacchi S, Murtas G. Human D-amino acid oxidase: structure, function, and regulation. Front Mol Biosci. 2018, 5:107. doi: 10.3389/fmolb.2018.00107. Review.

Pollegioni L, Sasabe J. Editorial: Bioscience of D-amino acid oxidase from biochemistry to pathophysiology. Front Mol Biosci. 2018, 5: 108. doi: 10.3389/fmolb.2018.00108. 

La Vitola P, Balducci C, Cerovic M, Santamaria G, Brandi E, Grandi F, Caldinelli L, Colombo L, Morgese MG, Trabace L, Pollegioni L, Albani D, Forloni G. Alpha-synuclein oligomers impair memory through glial cell activation and via Toll-like receptor 2. Brain Behav Immun. 2018, 69: 591-602. doi: 10.1016/j.bbi.2018.02.012. 

Cappelletti P, Tallarita E, Rabattoni V, Campomenosi P, Sacchi S, Pollegioni L. Proline oxidase controls proline, glutamate, and glutamine cellular concentrations in a U87 glioblastoma cell line. PLoS One. 2018, 13: e0196283. doi: 10.1371/journal.pone.0196283. 

Pollegioni L, Piubelli L, Molla G, Rosini E. D-amino acid oxidase-pLG72 interaction and D-serine modulation. Front Mol Biosci. 2018, 5: 3. doi: 10.3389/fmolb.2018.00003. Review.

Melis R, Rosini E, Pirillo V, Pollegioni L, Molla G. In vitro evolution of an L-amino acid deaminase active on L-1-naphthylalanine. Catal. Sci. Technol. 2018, 8: 5359-5367. doi: 10.1039/c8cy01380b.


Rosini E, Caldinelli L, Piubelli L. Assays of D-amino acid oxidase activity. Front Mol Biosci. 2018, 4:102. doi: 10.3389/fmolb.2017.00102. 

Tengattini S, Rinaldi F, Piubelli L, Kupfer T, Peters B, Bavaro T, Calleri E, Massolini G, Temporini C. Enterokinase monolithic bioreactor as an efficient tool for biopharmaceuticals preparation: on-line cleavage of fusion proteins and analytical characterization of released products. J Pharm Biomed Anal. 2018, 157: 10-19. doi: 10.1016/j.jpba.2018.05.005. 

Sacchi S, Cappelletti P, Murtas G. Biochemical properties of human D-amino acid oxidase variants and their potential significance in pathologies. Front Mol Biosci. 2018, 5: 55. doi: 10.3389/fmolb.2018.00055. Review.

Keller S, Punzo D, Cuomo M, Affinito O, Coretti L, Sacchi S, Florio E, Lembo F, Carella M, Copetti M, Cocozza S, Balu DT, Errico F, Usiello A, Chiariotti L. DNA methylation landscape of the genes regulating D-serine and D-aspartate metabolism in post-mortem brain from controls and subjects with schizophrenia. Sci Rep. 2018, 8: 10163. doi: 10.1038/s41598-018-28332-x. 

Pollegioni L, Mothet JP, Molla G. Special issue on "D-amino acids: biology in the mirror". Biochim Biophys Acta Proteins Proteom. 2018, 1866: 741-742. doi: 10.1016/j.bbapap.2018.04.009. 


Raboni S, Revtovich S, Demitri N, Giabbai B, Storici P, Cocconcelli C, Faggiano S, Rosini E, Pollegioni L, Galati S, Buschini A, Morozova E, Kulikova V, Nikulin A, Gabellieri E, Cioni P, Demidkina T, Mozzarelli A. Engineering methionine γ-lyase from Citrobacter freundii for anticancer activity. Biochim Biophys Acta Proteins Proteom. 2018, 1866:1260-1270. doi: 10.1016/j.bbapap.2018.09.011. 

Bernetti M, Rosini E, Mollica L, Masetti M, Pollegioni L, Recanatini M, Cavalli A. Binding residence time through scaled molecular dynamics: a prospective application to hDAAO inhibitors. J Chem Inf Model. 2018, 58: 2255-2265. doi: 10.1021/acs.jcim.8b00518. 

Vignali E, Tonin F, Pollegioni L, Rosini E. Characterization and use of a bacterial lignin peroxidase with an improved manganese-oxidative activity. Appl Microbiol Biotechnol. 2018, 102: 10579-10588. doi: 10.1007/s00253-018-9409-3. 

Berini F, Verce M, Ausec L, Rosini E, Tonin F, Pollegioni L, Mandić-Mulec I. Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens. Appl Microbiol Biotechnol. 2018, 102: 2425-2439. doi: 10.1007/s00253-018-8785-z. 

Murtas G, Caldinelli L, Cappelletti P, Sacchi S, Pollegioni L. Human D-amino acid oxidase: the inactive G183R variant. Biochim Biophys Acta Proteins Proteom. 2018, 1866:822-830. doi: 10.1016/j.bbapap.2017.12.007. 

Saviane A, Romoli O, Bozzato A, Freddi G, Cappelletti C, Rosini E, Cappellozza S, Tettamanti G, Sandrelli F. Intrinsic antimicrobial properties of silk spun by genetically modified silkworm strains. Transgenic Res. 2018, 27: 87-101. doi: 10.1007/s11248-018-0059-0. 


Ferreira JS, Papouin T, Ladépêche L, Yao A, Langlais VC, Bouchet D, Dulong J, Mothet JP, Sacchi S, Pollegioni L, Paoletti P, Oliet SHR, Groc L. Co-agonists differentially tune GluN2B-NMDA receptor trafficking at hippocampal synapses. Elife. 2017 Jun 9;6:e25492. doi: 10.7554/eLife.25492. PMID: 28598327; PMCID: PMC5466419.

Molla G, Melis R, Pollegioni L. Breaking the mirror: L-amino acid deaminase, a novel stereoselective biocatalyst. Biotechnol Adv. 2017, 35: 657-668. doi: 10.1016/j.biotechadv.2017.07.011. Review.

Murtas G, Sacchi S, Valentino M, Pollegioni L. Biochemical properties of human D-amino acid oxidase. Front Mol Biosci. 2017, 4: 88. doi: 10.3389/fmolb.2017.00088. 

Rinaldi F, Tengattini S, Calleri E, Bavaro T, Piubelli L, Pollegioni L, Massolini G, Temporini C. Application of a rapid HILIC-UV method for synthesis optimization and stability studies of immunogenic neo-glycoconjugates. J Pharm Biomed Anal. 2017, 144: 252-262. doi: 10.1016/j.jpba.2017.03.052. 

Cappelletti P, Binda E, Tunesi M, Albani D, Giordano C, Molla G, Pollegioni L. Recombinant human Tat-Hsp70-2: A tool for neuroprotection. Protein Expr Purif. 2017, 138: 18-24. doi: 10.1016/j.pep.2016.07.005. 

Sacchi S, Cappelletti P, Pirone L, Smaldone G, Pedone E, Pollegioni L. Elucidating the role of the pLG72 R30K substitution in schizophrenia susceptibility. FEBS Lett. 2017, 591: 646-655. doi: 10.1002/1873-3468.12585. 

Cassidy J, Bruen L, Rosini E, Molla G, Pollegioni L, Paradisi F. Engineering substrate promiscuity in halophilic alcohol dehydrogenase (HvADH2) by in silico design. PLoS One. 2017, 12: e0187482. doi: 10.1371/journal.pone.0187482. 

Rosini E, Melis R, Molla G, Tessaro D, Pollegioni L. Deracemization and stereoinversion of α-amino acids by L-amino acid deaminase. Adv Synth Catal. 2017, 359: 3773-3781.  doi: 10.1002/adsc.201700806.


Van Horn MR, Strasser A, Miraucourt LS, Pollegioni L, Ruthazer ES. The gliotransmitter D-serine promotes synapse maturation and axonal stabilization in vivo. J Neurosci. 2017, 37: 6277-6288. doi: 10.1523/JNEUROSCI.3158-16.2017. 

Casciello C, Tonin F, Berini F, Fasoli E, Marinelli F, Pollegioni L, Rosini E. A valuable peroxidase activity from the novel species Nonomuraea gerenzanensis growing on alkali lignin. Biotechnol Rep (Amst). 2017, 13: 49-57. doi: 10.1016/j.btre.2016.12.005. 

Berini F, Presti I, Beltrametti F, Pedroli M, Vårum KM, Pollegioni L, Sjöling S, Marinelli F. Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome. Microb Cell Fact. 2017, 16: 16. doi: 10.1186/s12934-017-0634-8.

Luks L, Maier MY, Sacchi S, Pollegioni L, Dietrich DR. Understanding renal nuclear protein accumulation: an in vitro approach to explain an in vivo phenomenon. Arch Toxicol. 2017, 91: 3599-3611. doi: 10.1007/s00204-017-1970-5. 

Tengattini S, Domínguez-Vega E, Temporini C, Bavaro T, Rinaldi F, Piubelli L, Pollegioni L, Massolini G, Somsen GW. Hydrophilic interaction liquid chromatography-mass spectrometry as a new tool for the characterization of intact semi-synthetic glycoproteins. Anal Chim Acta. 2017, 981: 94-105. doi: 10.1016/j.aca.2017.05.020. 

Tonin F, Vignali E, Pollegioni L, D'Arrigo P, Rosini E. A novel, simple screening method for investigating the properties of lignin oxidative activity. Enzyme Microb Technol. 2017, 96: 143-150. doi: 10.1016/j.enzmictec.2016.10.013. 

Nuzzo T, Sacchi S, Errico F, Keller S, Palumbo O, Florio E, Punzo D, Napolitano F, Copetti M, Carella M, Chiariotti L, Bertolino A, Pollegioni L, Usiello A. Decreased free D-aspartate levels are linked to enhanced D-aspartate oxidase activity in the dorsolateral prefrontal cortex of schizophrenia patients. NPJ Schizophr. 2017, 3:16. doi: 10.1038/s41537-017-0015-7. 

Sacchi S, Novellis V, Paolone G, Nuzzo T, Iannotta M, Belardo C, Squillace M, Bolognesi P, Rosini E, Motta Z, Frassineti M, Bertolino A, Pollegioni L, Morari M, Maione S, Errico F, Usiello A. Olanzapine, but not clozapine, increases glutamate release in the prefrontal cortex of freely moving mice by inhibiting D-aspartate oxidase activity. Sci Rep. 2017, 7: 46288. doi: 10.1038/srep46288. 

Luks L, Sacchi S, Pollegioni L, Dietrich DR. Novel insights into renal D-amino acid oxidase accumulation: propiverine changes DAAO localization and peroxisomal size in vivo. Arch Toxicol. 2017, 91: 427-437. doi: 10.1007/s00204-016-1685-z. 

Bavaro T, Tengattini S, Piubelli L, Mangione F, Bernardini R, Monzillo V, Calarota S, Marone P, Amicosante M, Pollegioni L, Temporini C, Terreni M. Glycosylation of recombinant antigenic proteins from Mycobacterium tuberculosis: in silico prediction of protein epitopes and ex vivo biological evaluation of new semi-synthetic glycoconjugates. Molecules. 2017, 22: 1081. doi: 10.3390/molecules22071081. 

Molla G. Competitive inhibitors unveil structure/function relationships in human D-amino acid oxidase. Front Mol Biosci. 2017, 4: 80. doi: 10.3389/fmolb.2017.00080. Review.


Sacchi S, Binelli G, Pollegioni L. G72 primate-specific gene: a still enigmatic element in psychiatric disorders. Cell Mol Life Sci. 2016, 73: 2029-2039. doi: 10.1007/s00018-016-2165-6. Review.

Tonin F, Melis R, Cordes A, Sanchez-Amat A, Pollegioni L, Rosini E. Comparison of different microbial laccases as tools for industrial uses. N Biotechnol. 2016, 33: 387-398. doi: 10.1016/j.nbt.2016.01.007. 

Meunier CN, Dallérac G, Le Roux N, Sacchi S, Levasseur G, Amar M, Pollegioni L, Mothet JP, Fossier P. D-serine and glycine differentially control neurotransmission during visual cortex critical period. PLoS One. 2016, 11: e0151233. doi: 10.1371/journal.pone.0151233. 

Tonin F, Rosini E, Piubelli L, Sanchez-Amat A, Pollegioni L. Different recombinant forms of polyphenol oxidase A, a laccase from Marinomonas mediterranea. Protein Expr Purif. 2016,123: 60-69. doi: 10.1016/j.pep.2016.03.011. 

Rosini E, D'Arrigo P, Pollegioni L. Demethylation of vanillic acid by recombinant LigM in a one-pot cofactor regeneration system. Catal. Sci. Technol. 2016, 6:7729-7737. doi: 10.1039/c6cy01402j.

Rosini E, Allegretti C, Melis R, Cerioli L, Conti G, Pollegioni L, D'Arrigo P. Cascade enzymatic cleavage of the β-O-4 linkage in a lignin model compound. Catal. Sci. Technol., 6:2195-2205. doi:10.1039/C5CY01591J. 


Motta P, Molla G, Pollegioni L, Nardini M. Structure-Function Relationships in L-Amino Acid Deaminase, a Flavoprotein Belonging to a Novel Class of Biotechnologically Relevant Enzymes. J Biol Chem. 2016, 291:10457-10475. doi: 10.1074/jbc.M115.703819. 

Birolo L, Sacchi S, Smaldone G, Molla G, Leo G, Caldinelli L, Pirone L, Eliometri P, Di Gaetano S, Orefice I, Pedone E, Pucci P, Pollegioni L. Regulating levels of the neuromodulator D-serine in human brain: structural insight into pLG72 and D-amino acid oxidase interaction. FEBS J. 2016, 283: 3353-3370. doi: 10.1111/febs.13809. 

Punzo D, Errico F, Cristino L, Sacchi S, Keller S, Belardo C, Luongo L, Nuzzo T, Imperatore R, Florio E, De Novellis V, Affinito O, Migliarini S, Maddaloni G, Sisalli MJ, Pasqualetti M, Pollegioni L, Maione S, Chiariotti L, Usiello A. Age-related changes in D-aspartate oxidase promoter methylation control extracellular D-aspartate levels and prevent precocious cell death during brain aging. J Neurosci. 2016, 36: 3064-3078. doi: 10.1523/JNEUROSCI.3881-15.2016. 

Pezzoli D, Tallarita E, Rosini E, Candiani G. Characterization and investigation of redox-sensitive liposomes for gene delivery. Methods Mol Biol. 2016,1445: 217-233. doi: 10.1007/978-1-4939-3718-9_14.