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Systems Biocatalysis: multi-enzymatic processes for the production
of high-value products

PI: Elena Rosini and Loredano Pollegioni


Filippo Molinari, Davide Miani, Erika Zangelmi





Not funded



Gianfranco GILARDI, Università degli studi di Torino, Torino, IT

Enzymes are widely employed for organic synthesis because of their smaller ecological footprint as compared to chemical processes and of their higher sustainability. Systems Biocatalysis is a promising approach aimed to assemble individual enzymes in vitro to develop multi-enzymatic cascades in a single reactor. In search of a sustainable biobased economy, entirely new non-natural pathways can be built to convert very low-value substrates to high-value products. 


The aim of this project is to use wheat bran-derived ferulic acid or lignin-derived vanillin as substrates to generate vanillin, cis,cis-muconic acid, adipic acid, and 2-pyrone-4,6-dicarboxylate (PDC), utilizing multi-enzymatic cascade processes. Lignin valorization would significantly enhance the economics of lignocellulose-based biorefineries. Moreover, there is great interest to convert wheat bran, an agriculture by-product obtained in large amounts in Italy, into added value products. 

Notably, the bioconversion processes are carried out using both recombinant enzymes and whole cell systems.

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