The Protein Factory 2.0
Welcome to a world of new solutions
The ProPla project
Proper waste management represents one of the most pressing challenges of the 21st century and plays a key role in sustainable development. Unsustainable use and disposal of plastics are causing persistent and widespread environmental contamination, in particular due to microplastics (MPs, 1 to < 1000 μm particles) - resulting from larger plastic - that may cause adverse effects in ecosystems and organisms.
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The project addresses the problem of MPs focusing on their recovery and valorization through a pre-competitive, multidisciplinary, and interdisciplinary approach. ProPla aims to develop an innovative biotechnological application to recover PET microplastics (microPET) from wastewater and exploit the power of protein engineering and systems biology approaches to generate a novel bacterial strain able to convert microPET into amino acids (plastic-eating activity) or high-value compounds (protein, lipid, chitin) through insects appropriately "modified." The goal is a paradigm shift, i.e., to enhance an environmental contaminant, potentially harmful even to human health, by converting it into a resource to produce goods useful to society, through a totally "green" approach.
Project objectives (starting: 1st of March 2023; duration: 36 months)
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Developing an innovative biotechnological application to recover PET microplastics (microPET) from wastewater.
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Generating a novel bacterial E. coli strain able to convert microPET into amino acids (plastic-eating activity), exploiting the power of protein engineering and systems biology approaches.
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Setting up a biological tool for a sustainable valorisation of microPET, colonizing the larval gut microbiota of the saprophagous insect Hermetia illucens (black soldier fly, BSF) with the engineered recombinant bacterial strain to provide the ability to degrade the microPET added to their diet and thus improve the value of the insect biomass.
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Reducing the negative environmental and public health outcomes generated by indiscriminate and inappropriate disposal practices, poor-quality treatments, and uncontrolled leaching of microPET.
The research team
The team is made up of experts from seven different research units involved in the study of plastic pollution, enzymatic engineering, systems biology and microbiology, entomology, economics, and the study of the life cycle of materials.
Meet the project partners!
The Protein Factory Lab - https://www.theproteinfactory2.it/
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University of Insubria
Loredano Pollegioni
Elena Rosini
Gianluca Molla
Caren Battaglia
Davide Miani
Invertebrate Biology Lab - https://www.dbsv.uninsubria.it/zoologia/
Gianluca Tettamanti
Daniele Bruno
Aurora Montali
Environmental Engineering group
Vincenzo Torretta
Fabio Conti
Elena Rada
Alberto Baltrocchi
Marco Carnevale Miino
Research Unit of Economics - www.uninsubria.it/dieco
Raffaello Seri
Giulia Pesaro
Elena Maggi
Anna Claudia Caspani
Andrea Vezzulli
Paolo Castelnovo
University of Milano-Bicocca
Chiara Damiani
Luca Brambilla
Alex Pessina
Marco Vanoni
Insect Physiology and Biotechnology Lab - https://bioscienzebio.unimi.it/gruppo/8
University of Milan
Silvia Caccia
Morena Casartelli
Maria Carmen Valoroso
Davis Roma
CNR-IRSA: Water Research Institute (Verbania) - https://www.irsa.cnr.it/wp/?page_id=376
Silvia Galafassi
Rosa Zullo